The present Studies were designed to investigate the morphology and stainability of the chicken spermatozoa. Semen samples were collected by abdominal massage from 10 cocks of Arbor, Acres strain (egg breed) and 10 cocks of white Cornish strain (meat breed). The semen samp1es were diluted with Sarker´s solution and were washed. Some of the semen smear slides were stained with seven differential stain methods and was compared with one another by light microscope. In addition to the staining already compared, the length of heads, middle pieces and tails of 400 spermatozoa of two chicken breed was measured with micrometer.
The results obtained from these, studies were as follows:
1. Eosin stain appeared to give good results than hematoxylin, pre-treated protease and eosin or hematoxylin stain, pre-treated protease and hematoxylin-eosin stain, carbol-fuchsin, stain and Giemsa 9 technique in differential staining of spermatozoal three portions and pre-treated protease and eosin stain appeared as good staining methods for middle piece of spermatozoa.
2. The averase length of chicken spermatozoa was 90.4±7.0㎛, and the average length of the head, middle piece and tail of spermatozoa was 13.0±0.5㎛, 3.8±0.2㎛ and 73.6±3.8㎛ lesoectively.
3. The average length of spermatozoa of Arbor Acres strain was 89.2±5.0㎛ and the average length of the head, middle piece and tail of spermatozoa was 12.9±0.5㎛, 3.8±0.2㎛ and 72.5±4.7㎛ respectively. The average length of spermatozoa of with Cornish was 91.6=3.0㎛ and the average length of the head, middle piece and tail of spermatozoa was 13.1±0.5㎛, 3.8±0.2㎛ and 74.7±2.8㎛ respectively.
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